1 January 2009 Multipoint fluorescence correlation spectroscopy with total internal reflection fluorescence microscope
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Abstract
We report simultaneous determination of diffusion coefficients at different points of a cell membrane using a multipoint fluorescence correlation spectroscopy (FCS) system. A system carrying seven detection areas in the evanescent field is achieved by using seven optical fibers on the image plane in the detection port of an objective-type total internal reflection FCS (TIR-FCS) system. Fluctuation of fluorescence intensity is monitored and evaluated using seven photomultiplier tubes (PMTs) and a newly constructed multichannel correlator. We demonstrate simultaneous-multipoint FCS, with a 3-μs time resolution, to investigate heterogeneous structures such as cell membranes and membrane-binding molecular dynamics near glass surfaces in live cells.
© (2009) Society of Photo-Optical Instrumentation Engineers (SPIE)
Yu Ohsugi, Yu Ohsugi, Masataka Kinjo, Masataka Kinjo, } "Multipoint fluorescence correlation spectroscopy with total internal reflection fluorescence microscope," Journal of Biomedical Optics 14(1), 014030 (1 January 2009). https://doi.org/10.1117/1.3080723 . Submission:
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