1 May 2009 Acoustic fingerprints of dye-labeled protein submicrosphere photoacoustic contrast agents
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Dye-labeled protein microspheres, submicron in size and capable of producing thermoelastically generated ultrasound in response to laser stimulation, are presented as contrast agents for photoacoustic imaging. Incident laser energy absorbed by fluorescein isothiocyanate (FITC)-labeled elastin submicrospheres results in thermoelastically generated sound production. Plotted A-line graphs reveal a distinctive morphology and a greater than two orders of magnitude increase in signal amplitude subsequent to converting FITC elastin into submicrospheres (despite a four orders of magnitude decrease in concentration). Evidence of nonlinearity and enhancement of ultrasound backscatter indicate a potential use in contrast-enhanced harmonic imaging. Photoacoustic and ultrasound imaging of FITC-elastin submicrospheres in a water-filled phantom vessel shows enhanced contrast at low concentration and clear delineation of the phantom vessel wall.
© (2009) Society of Photo-Optical Instrumentation Engineers (SPIE)
Michael A. McDonald, Michael A. McDonald, Ladislav Jankovic, Ladislav Jankovic, Khalid Shahzad, Khalid Shahzad, Michael Burcher, Michael Burcher, King C. P. Li, King C. P. Li, } "Acoustic fingerprints of dye-labeled protein submicrosphere photoacoustic contrast agents," Journal of Biomedical Optics 14(3), 034032 (1 May 2009). https://doi.org/10.1117/1.3155519 . Submission:

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