1 May 2009 Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy
Author Affiliations +
Abstract
Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, including dry mass production, density, and spatial distribution. We have applied DHM to study the dry mass production rate and the dry mass surface density in wild-type and mutant fission yeast cells. Our study demonstrates the applicability of DHM as a tool for label-free quantitative analysis of the cell cycle and opens the possibility for its use in high-throughput screening.
© (2009) Society of Photo-Optical Instrumentation Engineers (SPIE)
Benjamin Rappaz, Benjamin Rappaz, Elena Cano, Elena Cano, Tristan Colomb, Tristan Colomb, Jonas Kuhn, Jonas Kuhn, Christian D. Depeursinge, Christian D. Depeursinge, Viesturs Simanis, Viesturs Simanis, Pierre J. Magistretti, Pierre J. Magistretti, Pierre P. Marquet, Pierre P. Marquet, } "Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy," Journal of Biomedical Optics 14(3), 034049 (1 May 2009). https://doi.org/10.1117/1.3147385 . Submission:
JOURNAL ARTICLE
5 PAGES


SHARE
Back to Top