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1 May 2009 Feasibility of digitally stained multimodal confocal mosaics to simulate histopathology
Daniel S. Gareau
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Journal of Biomedical Optics, Vol. 14, Issue 3, 034050 (May 2009). https://doi.org/10.1117/1.3149853
Abstract
Fluorescence confocal mosaicing microscopy of tissue biopsies stained with acridine orange has been shown to accurately identify tumors and with an overall sensitivity of 96.6% and specificity of 89.2%. However, fluorescence shows only nuclear detail similar to hematoxylin in histopathology and does not show collagen or cytoplasm, which may provide necessary negative contrast information similar to eosin used in histopathology. Reflectance mode contrast is sensitive to collagen and cytoplasm without staining. To further improve sensitivity and specificity, digitally stained confocal mosaics combine confocal fluorescence and reflectance images in a multimodal pseudo-color image to mimic the appearance of histopathology with hematoxylin and eosin and facilitate the introduction of confocal microscopy into the clinical realm.
©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Daniel S. Gareau "Feasibility of digitally stained multimodal confocal mosaics to simulate histopathology," Journal of Biomedical Optics 14(3), 034050 (1 May 2009). https://doi.org/10.1117/1.3149853
Published: 1 May 2009
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Cited by 96 scholarly publications and 6 patents.
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KEYWORDS
Confocal microscopy
Luminescence
Reflectivity
Tumors
Collagen
Tissues
Surgery
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