Label-free imaging by stimulated parametric emission microscopy reveals a difference in hemoglobin distribution between live and fixed erythrocytes

Hieu M. Dang; Gen Omura; Toshiyuki Omano; Masatomo Yamagiwa; Shin'ichiro Kajiyama; Yasuyuki Ozeki; Kazuyoshi Itoh; Kiichi Fukui

doi:10.1117/1.3207151

1 July 2009 Label-free imaging by stimulated parametric emission microscopy reveals a difference in hemoglobin distribution between live and fixed erythrocytes

Hieu M. Dang, Gen Omura, Toshiyuki Omano, Masatomo Yamagiwa, Shin'ichiro Kajiyama, Yasuyuki Ozeki, Kazuyoshi Itoh, Kiichi Fukui

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Journal of Biomedical Optics, Vol. 14, Issue 4, 040506 (July 2009). https://doi.org/10.1117/1.3207151

Abstract

We demonstrate that stimulated parametric emission (SPE) microscopy enables label-free, 3-D visualization of internal hemoglobin distribution of live mouse and chicken erythrocytes with high sensitivity. Change in hemoglobin distribution in chicken erythrocytes before and after ethanol fixation is clearly visualized.

©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Hieu M. Dang, Gen Omura, Toshiyuki Omano, Masatomo Yamagiwa, Shin'ichiro Kajiyama, Yasuyuki Ozeki, Kazuyoshi Itoh, and Kiichi Fukui "Label-free imaging by stimulated parametric emission microscopy reveals a difference in hemoglobin distribution between live and fixed erythrocytes," Journal of Biomedical Optics 14(4), 040506 (1 July 2009). https://doi.org/10.1117/1.3207151

Published: 1 July 2009

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