Stress differences via spectral shifts that arise among failed, strained, and undamaged regions of bone can be determined using Raman spectroscopy and double-notch specimens. A double-notch specimen is a model in which the early stages of fracture can be examined. At four-point bending, fracture occurs at one of the notches. Tissue near each notch is representative of bone in a state either directly before or after bone failure. Raman images are acquired among three regions: control, strained (root of unbroken notch), and failed (root of fractured notch). The center of gravities (CGs), a way to monitor wavenumber shifts, of the phosphate v1 band are calculated. A PO4-3 v1 band shift most likely corresponds to a change in spacing between phosphate cations and anions. This spectral shift is converted into stress values using the dv/dP coefficient, determined by applying known pressures/stresses and measuring the change in position of the PO4-3 v1 band. In comparison to control regions, the residual stress in strained and failed regions is significantly higher (p=0.0425 and p=0.0169, respectively). In strained regions, residual stress is concentrated near the corners of the unbroken notch, whereas in failed regions the high stresses are confined near the edge of the fracture.