1 September 2009 Profile of new green fluorescent protein transgenic Jinhua pigs as an imaging source
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Abstract
Animal imaging sources have become an indispensable material for biological sciences. Specifically, gene-encoded biological probes serve as stable and high-performance tools to visualize cellular fate in living animals. We use a somatic cell cloning technique to create new green fluorescent protein (GFP)-expressing Jinhua pigs with a miniature body size, and characterized the expression profile in various tissues/organs and ex vivo culture conditions. The born GFP-transgenic pig demonstrate an organ/tissue-dependent expression pattern. Strong GFP expression is observed in the skeletal muscle, pancreas, heart, and kidney. Regarding cellular levels, bone-marrow-derived mesenchymal stromal cells, hepatocytes, and islet cells of the pancreas also show sufficient expression with the unique pattern. Moreover, the cloned pigs demonstrate normal growth and fertility, and the introduced GFP gene is stably transmitted to pigs in subsequent generations. The new GFP-expressing Jinhua pigs may be used as new cellular/tissue light resources for biological imaging in preclinical research fields such as tissue engineering, experimental regenerative medicine, and transplantation.
© (2009) Society of Photo-Optical Instrumentation Engineers (SPIE)
Tatsuo Kawarasaki, Tatsuo Kawarasaki, Kazuhiko Uchiyama, Kazuhiko Uchiyama, Atsushi Hirao, Atsushi Hirao, Sadahiro Azuma, Sadahiro Azuma, Masayoshi Otake, Masayoshi Otake, Masatoshi Shibata, Masatoshi Shibata, Seiko Tsuchiya, Seiko Tsuchiya, Shin Enosawa, Shin Enosawa, Koichi Takeuchi, Koichi Takeuchi, Kenjiro Konno, Kenjiro Konno, Yoji Hakamata, Yoji Hakamata, Hiroyuki Yoshino, Hiroyuki Yoshino, Takuya Wakai, Takuya Wakai, Shigeo Ookawara, Shigeo Ookawara, Hozumi Tanaka, Hozumi Tanaka, Eiji Kobayashi, Eiji Kobayashi, Takashi Murakami, Takashi Murakami, } "Profile of new green fluorescent protein transgenic Jinhua pigs as an imaging source," Journal of Biomedical Optics 14(5), 054017 (1 September 2009). https://doi.org/10.1117/1.3241985 . Submission:
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