1 September 2009 Specific immobilization of influenza A virus on GaAs (001) surface
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Abstract
In the quest for the development of an all-optical biosensor for rapid detection and typing of viral pathogens, we investigate biosensing architectures that take advantage of strong photoluminescence emission from III-V quantum semiconductors (QS). One of the key elements in the development of such a biosensor is the ability to attach various analytes to GaAs-a material of choice for capping III-V QS of our interest. We report on the study of biofunctionalization of GaAs (001) with polyethylene-glycol (PEG) thiols and the successful immobilization of influenza A virus. A diluted solution of biotinylated PEG thiols in OH-terminated PEG thiols is used to form a network of sites for the attachment of neutravidin. Biotinylated polyclonal influenza A antibodies are applied to investigate the process of the immobilization of inactivated influenza A virus. The successful immobilization is demonstrated using atomic force microscopy and fluorescence microscopy measurements.
© (2009) Society of Photo-Optical Instrumentation Engineers (SPIE)
Valerie Duplan, Valerie Duplan, Yannick Miron, Yannick Miron, Eric H. Frost, Eric H. Frost, Michel Grandbois, Michel Grandbois, Jan J. Dubowski, Jan J. Dubowski, } "Specific immobilization of influenza A virus on GaAs (001) surface," Journal of Biomedical Optics 14(5), 054042 (1 September 2009). https://doi.org/10.1117/1.3251057 . Submission:
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