1 November 2009 Analysis of bacterial chemotactic response using dynamic laser speckle
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Abstract
Chemotaxis has a meaningful role in several fields, such as microbial physiology, medicine and biotechnology. We present a new application of dynamic laser speckle (or biospeckle) to detect different degrees of bacterial motility during chemotactic response experiments. Encouraging results showed different bacterial dynamic responses due to differences in the hardness of the support in the swarming plates. We compare this method to a conventional technique that uses white light. Both methods showed to be analogous and, in some cases, complementary. The results suggest that biospeckle processed images can be used as an alternative method to evaluate bacterial chemotactic response and can supply additional information about the bacterial motility in different areas of the swarm plate assay that might be useful for biological analysis.
© (2009) Society of Photo-Optical Instrumentation Engineers (SPIE)
Silvia Murialdo, Silvia Murialdo, Gonzalo Hernán Sendra, Gonzalo Hernán Sendra, Lucia Passoni, Lucia Passoni, Ricardo Arizaga, Ricardo Arizaga, Jorge Froilán Gonzalez, Jorge Froilán Gonzalez, Héctor Jorge Rabal, Héctor Jorge Rabal, Marcelo Trivi, Marcelo Trivi, } "Analysis of bacterial chemotactic response using dynamic laser speckle," Journal of Biomedical Optics 14(6), 064015 (1 November 2009). https://doi.org/10.1117/1.3262608 . Submission:
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