1 November 2009 Is there a difference between T- and B-lymphocyte morphology?
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We characterize T- and B-lymphocytes from several donors, determining cell diameter, ratio of nucleus to cell diameter, and refractive index of the nucleus and cytoplasm for each individual cell. We measure light-scattering profiles with a scanning flow cytometer and invert the signals using a coated sphere as an optical model of the cell and by relying on a global optimization technique. The main difference in morphology of T- and B-lymphocytes is found to be the larger mean diameters of the latter. However, the difference is smaller than the natural biological variability of a single cell. We propose nuclear inhomogeneity as a possible reason for the deviation of measured light-scattering profiles from real lymphocytes from those obtained from the coated sphere model.
© (2009) Society of Photo-Optical Instrumentation Engineers (SPIE)
Dmitry I. Strokotov, Dmitry I. Strokotov, Maxim A. Yurkin, Maxim A. Yurkin, Konstantin V. Gilev, Konstantin V. Gilev, Dirk R. Van Bockstaele, Dirk R. Van Bockstaele, Alfons G. Hoekstra, Alfons G. Hoekstra, Nikolay Rubtsov, Nikolay Rubtsov, Valerii Pavlovich Maltsev, Valerii Pavlovich Maltsev, } "Is there a difference between T- and B-lymphocyte morphology?," Journal of Biomedical Optics 14(6), 064036 (1 November 2009). https://doi.org/10.1117/1.3275471 . Submission:

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