1 January 2010 Imaging morphogenesis of Candida albicans during infection in a live animal
Author Affiliations +
J. of Biomedical Optics, 15(1), 010504 (2010). doi:10.1117/1.3290243
Abstract
Candida albicans is an opportunistic human fungal pathogen that requires an intact host immune response to prevent disease. Thus, studying host-pathogen interactions is critical to understanding and preventing this disease. We report a new model infection system in which ongoing C. albicans infections can be imaged at high spatial resolution in the ears of living mice. Intradermal inoculation into mouse ears with a C. albicans strain expressing green fluorescent protein results in systemic C. albicans infection that can be imaged in vivo using confocal microscopy. We observed filamentous growth of the organism in vivo as well as formation of microabscesses. This model system will allow us to gain significant new information about C. albicans pathogenesis through studies of host-C. albicans interactions in the native environment.
Soumya Mitra, Kristy Dolan, Thomas H. Foster, Melanie Wellington, "Imaging morphogenesis of Candida albicans during infection in a live animal," Journal of Biomedical Optics 15(1), 010504 (1 January 2010). http://dx.doi.org/10.1117/1.3290243
Submission: Received ; Accepted
JOURNAL ARTICLE
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KEYWORDS
Ear

In vivo imaging

Systems modeling

Yeast

Luminescence

Confocal microscopy

Animal model studies

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