1 March 2010 Multimodal nonlinear optical imaging of atherosclerotic plaque development in myocardial infarction-prone rabbits
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J. of Biomedical Optics, 15(2), 020501 (2010). doi:10.1117/1.3353960
Abstract
Label-free imaging of bulk arterial tissue is demonstrated using a multimodal nonlinear optical microscope based on a photonic crystal fiber and a single femtosecond oscillator operating at 800 nm. Colocalized imaging of extracellular elastin fibers, fibrillar collagen, and lipid-rich structures within aortic tissue obtained from atherosclerosis-prone myocardial infarction-prone Watanabe heritable hyperlipidemic (WHHLMI) rabbits is demonstrated through two-photon excited fluorescence, second harmonic generation, and coherent anti-Stokes Raman scattering, respectively. These images are shown to differentiate healthy arterial wall, early atherosclerotic lesions, and advanced plaques. Clear pathological changes are observed in the extracellular matrix of the arterial wall and correlated with progression of atherosclerotic disease as represented by the age of the WHHLMI rabbits.
Alex C. T. Ko, Andrew Ridsdale, Michael S. D. Smith, Leila B. Mostaço-Guidolin, Mark D. Hewko, Adrian F. Pegoraro, Elicia M. Kohlenberg, Bernhard J. Schattka, Masashi Shiomi, Albert Stolow, Michael G. Sowa, "Multimodal nonlinear optical imaging of atherosclerotic plaque development in myocardial infarction-prone rabbits," Journal of Biomedical Optics 15(2), 020501 (1 March 2010). http://dx.doi.org/10.1117/1.3353960
Submission: Received ; Accepted
JOURNAL ARTICLE
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KEYWORDS
Collagen

Microscopes

Femtosecond phenomena

Nonlinear optics

Objectives

CARS tomography

Tissues

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