1 May 2010 Imaging targeted-agent binding in vivo with two probes
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J. of Biomedical Optics, 15(3), 030513 (2010). doi:10.1117/1.3449109
Abstract
An approach to quantitatively image targeted-agent binding rate in vivo is demonstrated with dual-probe injection of both targeted and nontargeted fluorescent dyes. Images of a binding rate constant are created that reveal lower than expected uptake of epidermal growth factor in an orthotopic xenograft pancreas tumor (2.3×10−5 s−1), as compared to the normal pancreas (3.4×10−5 s−1). This approach allows noninvasive assessment of tumor receptor targeting in vivo to determine the expected contrast, spatial localization, and efficacy in therapeutic agent delivery.
Brian W. Pogue, Kimberley S. Samkoe, Shannon K. Hextrum, Julia A. O'Hara, Michael Jermyn, Subhadra Srinivasan, Tayyaba Hasan, "Imaging targeted-agent binding in vivo with two probes," Journal of Biomedical Optics 15(3), 030513 (1 May 2010). http://dx.doi.org/10.1117/1.3449109
Submission: Received ; Accepted
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KEYWORDS
Tumors

In vivo imaging

Pancreas

Tissues

Luminescence

Receptors

Plasma

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