1 July 2010 Direct detection of aptamer-thrombin binding via surface-enhanced Raman spectroscopy
Author Affiliations +
J. of Biomedical Optics, 15(4), 047006 (2010). doi:10.1117/1.3465594
Abstract
In this study, we exploit the sensitivity offered by surface-enhanced Raman scattering (SERS) for the direct detection of thrombin using the thrombin-binding aptamer (TBA) as molecular receptor. The technique utilizes immobilized silver nanoparticles that are functionalized with thiolated thrombin-specific binding aptamer, a 15-mer (5'-GGTTGGTGTGGTTGG-3') quadruplex forming oligonucleotide. In addition to the Raman vibrational bands corresponding to the aptamer and blocking agent, new peaks (mainly at 1140, 1540, and 1635 cm−1) that are characteristic of the protein are observed upon binding of thrombin. These spectral changes are not observed when the aptamer-nanoparticle assembly is exposed to a nonbinding protein such as bovine serum albumin (BSA). This methodology could be further used for the development of label-free biosensors for direct detection of proteins and other molecules of interest for which aptamers are available.
Cynthia V. Pagba, Stephen M. Lane, Hansang Cho, Sebastian Wachsmann-Hogiu, "Direct detection of aptamer-thrombin binding via surface-enhanced Raman spectroscopy," Journal of Biomedical Optics 15(4), 047006 (1 July 2010). http://dx.doi.org/10.1117/1.3465594
Submission: Received ; Accepted
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KEYWORDS
Surface enhanced Raman spectroscopy

Nanoparticles

Molecules

Proteins

Raman spectroscopy

Metals

Receptors

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