1 November 2010 Localization capability and limitation of electron-multiplying charge-coupled, scientific complementary metal-oxide semiconductor, and charge-coupled devices for superresolution imaging
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J. of Biomedical Optics, 15(6), 066005 (2010). doi:10.1117/1.3505017
Abstract
Localization of a single fluorescent molecule is required in a number of superresolution imaging techniques for visualizing biological structures at cellular and subcellular levels. The localization capability and limitation of low-light detectors are critical for such a purpose. We present an updated evaluation on the performance of three typical low-light detectors, including a popular electron-multiplying CCD (EMCCD), a newly developed scientific CMOS (sCMOS), and a representative cooled CCD, for superresolution imaging. We find that under some experimental accessible conditions, the sCMOS camera shows a competitive and even better performance than the EMCCD camera, which has long been considered the detector of choice in the field of superresolution imaging.
Tingwei Quan, Shaoqun Zeng, Zhenli Huang, "Localization capability and limitation of electron-multiplying charge-coupled, scientific complementary metal-oxide semiconductor, and charge-coupled devices for superresolution imaging," Journal of Biomedical Optics 15(6), 066005 (1 November 2010). http://dx.doi.org/10.1117/1.3505017
Submission: Received ; Accepted
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KEYWORDS
Sensors

Cameras

Electron multiplying charge coupled devices

Molecules

Super resolution

CCD cameras

Signal detection

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