1 November 2010 Dual-trap Raman tweezers for probing dynamics and heterogeneity of interacting microbial cells
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J. of Biomedical Optics, 15(6), 067008 (2010). doi:10.1117/1.3526357
Abstract
We report on development of dual-trap Raman tweezers for monitoring cellular dynamics and heterogeneity of interacting living cells suspended in a liquid medium. Dual-beam optical tweezers were combined with Raman spectroscopy, which allows capturing two cells that are in direct contact or closely separated by a few micrometers and simultaneously acquiring their Raman spectra with an imaging CCD spectrograph. As a demonstration, we recorded time-lapse Raman spectra of budding yeast cells held in dual traps for over 40 min to monitor the dynamic growth in a nutrient medium. We also monitored two germinating Bacillus spores after the initiation with L-alanine and observed their heterogeneity in the release of CaDPA under identical microenvironment.
Yan Li, Guiwen Wang, Hui-lu Yao, Junxian Liu, Yong-qing Li, "Dual-trap Raman tweezers for probing dynamics and heterogeneity of interacting microbial cells," Journal of Biomedical Optics 15(6), 067008 (1 November 2010). http://dx.doi.org/10.1117/1.3526357
Submission: Received ; Accepted
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KEYWORDS
Raman spectroscopy

Particles

CCD cameras

Optical tweezers

Yeast

Proteins

Spectrographs

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