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1 January 2010 Quantitative phase microscopy of articular chondrocyte dynamics by wide-field digital interferometry
Natan Tzvi Shaked, John D. Finan, Farshid Guilak, Adam P. Wax
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Journal of Biomedical Optics, Vol. 15, Issue 1, 010505 (January 2010). https://doi.org/10.1117/1.3290242
Abstract
We experimentally implement label-free phase microscopy using wide-field digital interferometry (WFDI) techniques to retrieve quantitative volumetric data of articular chondrocyte dynamics. Using the scanless interferometric system, we visualize chondrocyte swelling and bursting induced by hypo-osmotic pressure. Reconstructed images are obtained by an efficient digital process. We use the resulting images to calculate quantitative temporal-spatial morphological parameters of the cell, with the observed dynamics limited only by the true frame rate of the camera. To show the utility of WFDI in recording articular chondrocyte dynamics, we also provide an experimental comparison of WFDI and differential interference contrast microscopy.
©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Natan Tzvi Shaked, John D. Finan, Farshid Guilak, and Adam P. Wax "Quantitative phase microscopy of articular chondrocyte dynamics by wide-field digital interferometry," Journal of Biomedical Optics 15(1), 010505 (1 January 2010). https://doi.org/10.1117/1.3290242
Published: 1 January 2010
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Cited by 51 scholarly publications and 1 patent.
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KEYWORDS
Microscopy
Interferometry
Digital image correlation
Video
Visualization
Wavefronts
Cameras
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