1 January 2010 In vivo high-resolution structural imaging of large arteries in small rodents using two-photon laser scanning microscopy
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Abstract
In vivo (molecular) imaging of the vessel wall of large arteries at subcellular resolution is crucial for unraveling vascular pathophysiology. We previously showed the applicability of two-photon laser scanning microscopy (TPLSM) in mounted arteries ex vivo. However, in vivo TPLSM has thus far suffered from in-frame and between-frame motion artifacts due to arterial movement with cardiac and respiratory activity. Now, motion artifacts are suppressed by accelerated image acquisition triggered on cardiac and respiratory activity. In vivo TPLSM is performed on rat renal and mouse carotid arteries, both surgically exposed and labeled fluorescently (cell nuclei, elastin, and collagen). The use of short acquisition times consistently limit in-frame motion artifacts. Additionally, triggered imaging reduces between-frame artifacts. Indeed, structures in the vessel wall (cell nuclei, elastic laminae) can be imaged at subcellular resolution. In mechanically damaged carotid arteries, even the subendothelial collagen sheet (~1 μm) is visualized using collagen-targeted quantum dots. We demonstrate stable in vivo imaging of large arteries at subcellular resolution using TPLSM triggered on cardiac and respiratory cycles. This creates great opportunities for studying (diseased) arteries in vivo or immediate validation of in vivo molecular imaging techniques such as magnetic resonance imaging (MRI), ultrasound, and positron emission tomography (PET).
© (2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
Remco T. A. Megens, Remco T. A. Megens, S. Reitsma, S. Reitsma, Lenneke Prinzen, Lenneke Prinzen, Mirjam G. A. oude Egbrink, Mirjam G. A. oude Egbrink, Wim Engels, Wim Engels, Peter J. A. Leenders, Peter J. A. Leenders, Ellen J. L. Brunenberg, Ellen J. L. Brunenberg, Koen D. Reesink, Koen D. Reesink, Ben J. A. Janssen, Ben J. A. Janssen, Bart M. ter Haar Romeny, Bart M. ter Haar Romeny, Dick W. Slaaf, Dick W. Slaaf, Marc A. M. J. Van Zandvoort, Marc A. M. J. Van Zandvoort, } "In vivo high-resolution structural imaging of large arteries in small rodents using two-photon laser scanning microscopy," Journal of Biomedical Optics 15(1), 011108 (1 January 2010). https://doi.org/10.1117/1.3281672 . Submission:
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