1 January 2010 Screening small-molecule compound microarrays for protein ligands without fluorescence labeling with a high-throughput scanning microscope
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Abstract
We describe a high-throughput scanning optical microscope for detecting small-molecule compound microarrays on functionalized glass slides. It is based on measurements of oblique-incidence reflectivity difference and employs a combination of a y-scan galvometer mirror and an x-scan translation stage with an effective field of view of 2 cm×4 cm. Such a field of view can accommodate a printed small-molecule compound microarray with as many as 10,000 to 20,000 targets. The scanning microscope is capable of measuring kinetics as well as endpoints of protein-ligand reactions simultaneously. We present the experimental results on solution-phase protein reactions with small-molecule compound microarrays synthesized from one-bead, one-compound combinatorial chemistry and immobilized on a streptavidin-functionalized glass slide.
© (2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
Yiyan Fei, James P. Landry, Yungshin Sun, Xiangdong Zhu, Xiaobing Wang, Juntao Luo, Chun-yi Wu, Kit S. Lam, "Screening small-molecule compound microarrays for protein ligands without fluorescence labeling with a high-throughput scanning microscope," Journal of Biomedical Optics 15(1), 016018 (1 January 2010). https://doi.org/10.1117/1.3309743 . Submission:
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