1 January 2010 Screening small-molecule compound microarrays for protein ligands without fluorescence labeling with a high-throughput scanning microscope
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Abstract
We describe a high-throughput scanning optical microscope for detecting small-molecule compound microarrays on functionalized glass slides. It is based on measurements of oblique-incidence reflectivity difference and employs a combination of a y-scan galvometer mirror and an x-scan translation stage with an effective field of view of 2 cm×4 cm. Such a field of view can accommodate a printed small-molecule compound microarray with as many as 10,000 to 20,000 targets. The scanning microscope is capable of measuring kinetics as well as endpoints of protein-ligand reactions simultaneously. We present the experimental results on solution-phase protein reactions with small-molecule compound microarrays synthesized from one-bead, one-compound combinatorial chemistry and immobilized on a streptavidin-functionalized glass slide.
© (2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
Yiyan Fei, Yiyan Fei, James P. Landry, James P. Landry, Yungshin Sun, Yungshin Sun, Xiangdong Zhu, Xiangdong Zhu, Xiaobing Wang, Xiaobing Wang, Juntao Luo, Juntao Luo, Chun-yi Wu, Chun-yi Wu, Kit S. Lam, Kit S. Lam, } "Screening small-molecule compound microarrays for protein ligands without fluorescence labeling with a high-throughput scanning microscope," Journal of Biomedical Optics 15(1), 016018 (1 January 2010). https://doi.org/10.1117/1.3309743 . Submission:
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