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1 January 2010 Highly sensitive optical detection of specific protein in breast cancer cells using microstructured fiber in extremely low sample volume
Saraswathi Padmanabhan, V. K. Shinoj, Murukeshan Vadakke Matham, Parasuraman Padmanabhan
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Journal of Biomedical Optics, Vol. 15, Issue 1, 017005 (January 2010). https://doi.org/10.1117/1.3302810
Abstract
A simple optical method using hollow-core photonic crystal fiber for protein detection has been described. In this study, estrogen receptor (ER) from a MCF-7 breast carcinoma cell lysates immobilized inside a hollow-core photonic crystal fiber was detected using anti-ER primary antibody with either AlexaTM Fluor 488 (green fluorescent dye) or 555 (red Fluorescent dye) labeled Goat anti-rabbit IgG as the secondary antibody. The fluorescence fingerprints of the ERα protein were observed under fluorescence microscope, and its optical characteristics were analyzed. The ERα protein detection by this proposed method is based on immuno binding from sample volume as low as 50 nL. This method is expected to offer great potential as a biosensor for medical diagnostics and therapeutics applications.
©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Saraswathi Padmanabhan, V. K. Shinoj, Murukeshan Vadakke Matham, and Parasuraman Padmanabhan "Highly sensitive optical detection of specific protein in breast cancer cells using microstructured fiber in extremely low sample volume," Journal of Biomedical Optics 15(1), 017005 (1 January 2010). https://doi.org/10.1117/1.3302810
Published: 1 January 2010
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Cited by 44 scholarly publications and 1 patent.
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KEYWORDS
Proteins
Photonic crystal fibers
Breast cancer
Luminescence
Microscopes
Sensors
Biosensors
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