Hardware implementation algorithm and error analysis of high-speed fluorescence lifetime sensing systems using center-of-mass method

Day-Uei Li; Bruce R. Rae; Robin Andrews; Jochen Arlt; Robert K. Henderson

doi:10.1117/1.3309737

1 January 2010 Hardware implementation algorithm and error analysis of high-speed fluorescence lifetime sensing systems using center-of-mass method

Day-Uei Li, Bruce R. Rae, Robin Andrews, Jochen Arlt, Robert K. Henderson

Author Affiliations +

Journal of Biomedical Optics, Vol. 15, Issue 1, 017006 (January 2010). https://doi.org/10.1117/1.3309737

Abstract

A new, simple, high-speed, and hardware-only integration-based fluorescence-lifetime-sensing algorithm using a center-of-mass method (CMM) is proposed to implement lifetime calculations, and its signal-to-noise-ratio based on statistics theory is also deduced. Compared to the commonly used iterative least-squares method or the maximum-likelihood-estimation-based, general purpose fluorescence lifetime imaging microscopy (FLIM) analysis software, the proposed hardware lifetime calculation algorithm with CMM offers direct calculation of fluorescence lifetime based on the collected photon counts and timing information provided by in-pixel circuitry and therefore delivers faster analysis for real-time applications, such as clinical diagnosis. A real-time hardware implementation of this CMM FLIM algorithm suitable for a single-photon avalanche diode array in CMOS imaging technology is now proposed for implementation on field-programmable gate array. The performance of the proposed methods has been tested on Fluorescein, Coumarin 6, and 1,8-anilinonaphthalenesulfonate in water/methanol mixture.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Day-Uei Li, Bruce R. Rae, Robin Andrews, Jochen Arlt, and Robert K. Henderson "Hardware implementation algorithm and error analysis of high-speed fluorescence lifetime sensing systems using center-of-mass method," Journal of Biomedical Optics 15(1), 017006 (1 January 2010). https://doi.org/10.1117/1.3309737

Published: 1 January 2010

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
10 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

CITATIONS

Cited by 54 scholarly publications and 4 patents.

Explore citations on Lens.org

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Field programmable gate arrays

Fluorescence lifetime imaging

Error analysis

Sensing systems

Photon counting

Lithium

Show All Keywords

RELATED CONTENT

High speed multichannel time correlated single photon counting electronics based...
Proceedings of SPIE (May 29 2013)

Strong and weak stability of bipartite ranking algorithms
Proceedings of SPIE (July 19 2013)

Fluorescence lifetime imaging microscopy using a streak camera
Proceedings of SPIE (February 28 2014)

Method for accurate quantitation of background tissue optical properties in...
Proceedings of SPIE (March 04 2015)

A 65k pixel, 150k frames per second camera with global...
Proceedings of SPIE (May 15 2014)

Mechanism of two photon fluorescence increment via cross linked bovine...
Proceedings of SPIE (September 27 2012)

Subscribe to Digital Library

Receive Erratum Email Alert

Show All Keywords

Keywords/Phrases

Search In:

Publication Years