Wide-field four-channel fluorescence imager for biological applications

Madhuri M. Thakur; Dmitry G. Melnik; Heather Barnett; Kevin Daly; Christine H. Moran; Wei-Shun Chang; Stephan Link; C. Theodore Bucher Jr.; Carter Kittrell; Robert F. Curl

doi:10.1117/1.3374052

1 March 2010 Wide-field four-channel fluorescence imager for biological applications

Madhuri M. Thakur, Dmitry G. Melnik, Heather Barnett, Kevin Daly, Christine H. Moran, Wei-Shun Chang, Stephan Link, C. Theodore Bucher Jr., Carter Kittrell, Robert F. Curl

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Journal of Biomedical Optics, Vol. 15, Issue 2, 026016 (March 2010). https://doi.org/10.1117/1.3374052

Abstract

A wide-field four-channel fluorescence imager has been developed. The instrument uses four expanded laser beams to image a large section (6 mm×9 mm). An object can be sequentially illuminated with any combination of 408-, 532-, 658-, and 784-nm lasers for arbitrary (down to 1 ms) exposure times for each laser. Just two notch filters block scattered light from all four lasers. The design approach described here offers great flexibility in treatment of objects, very good sensitivity, and a wide field of view at low cost. There appears to be no commercial instrument capable of simultaneous fluorescence imaging of a wide field of view with four-laser excitation. Some possible applications are following events such as flow and mixing in microchannel systems, the transmission of biological signals across a culture, and following simulations of biological membrane diffusion. It can also be used in DNA sequencing by synthesis to follow the progress of the photolytic removal of dye and terminator. Without utilizing its time resolution, it can be used to obtain four independent images of a single tissue section stained with four targeting agents, with each coupled to a different dye matching one of the lasers.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Madhuri M. Thakur, Dmitry G. Melnik, Heather Barnett, Kevin Daly, Christine H. Moran, Wei-Shun Chang, Stephan Link, C. Theodore Bucher Jr., Carter Kittrell, and Robert F. Curl "Wide-field four-channel fluorescence imager for biological applications," Journal of Biomedical Optics 15(2), 026016 (1 March 2010). https://doi.org/10.1117/1.3374052

Published: 1 March 2010

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