1 March 2010 Nonlinear multicontrast microscopy of hematoxylin-and-eosin-stained histological sections
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Abstract
Imaging hematoxylin-and-eosin-stained cancerous histological sections with multicontrast nonlinear excitation fluorescence, second- and third-harmonic generation (THG) microscopy reveals cellular structures with extremely high image contrast. Absorption and fluorescence spectroscopy together with second hyperpolarizability measurements of the dyes shows that strong THG appears due to neutral hemalum aggregation and is subsequently enhanced by interaction with eosin. Additionally, fluorescence lifetime imaging microscopy reveals eosin fluorescence quenching by hemalums, showing better suitability of only eosin staining for fluorescence microscopy. Multicontrast nonlinear microscopy has the potential to differentiate between cancerous and healthy tissue at a single cell level.
© (2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
Adam E. Tuer, Adam E. Tuer, Danielle Tokarz, Danielle Tokarz, Nicole Prent, Nicole Prent, Richard Cisek, Richard Cisek, Jennifer Alami, Jennifer Alami, Daniel J. Dumont, Daniel J. Dumont, Ludmilla Bakueva, Ludmilla Bakueva, John A. Rowlands, John A. Rowlands, Virginijus Barzda, Virginijus Barzda, } "Nonlinear multicontrast microscopy of hematoxylin-and-eosin-stained histological sections," Journal of Biomedical Optics 15(2), 026018 (1 March 2010). https://doi.org/10.1117/1.3382908 . Submission:
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