1 March 2010 Micromirror-scanned dual-axis confocal microscope utilizing a gradient-index relay lens for image guidance during brain surgery
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Abstract
A fluorescence confocal microscope incorporating a 1.8-mm-diam gradient-index relay lens is developed for in vivo histological guidance during resection of brain tumors. The microscope utilizes a dual-axis confocal architecture to efficiently reject out-of-focus light for high-contrast optical sectioning. A biaxial microelectromechanical system (MEMS) scanning mirror is actuated at resonance along each axis to achieve a large field of view with low-voltage waveforms. The unstable Lissajous scan, which results from actuating the orthogonal axes of the MEMS mirror at highly disparate resonance frequencies, is optimized to fully sample 500×500 pixels at two frames per second. Optically sectioned fluorescence images of brain tissues are obtained in living mice to demonstrate the utility of this microscope for image-guided resections.
© (2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
Jonathan T. Liu, Michael J. Mandella, Nathan O. Loewke, Henry Haeberle, Hyejun Ra, Wibool Piyawattanametha, Olav D. Solgaard, Gordon S. Kino, Christopher H. Contag, "Micromirror-scanned dual-axis confocal microscope utilizing a gradient-index relay lens for image guidance during brain surgery," Journal of Biomedical Optics 15(2), 026029 (1 March 2010). https://doi.org/10.1117/1.3386055 . Submission:
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