Multiphoton microscopy with near infrared contrast agents

Siavash Yazdanfar; Chulmin Joo; Chun Zhan; Mikhail Berezin; Walter J. Akers; Samuel Achilefu

doi:10.1117/1.3420209

1 May 2010 Multiphoton microscopy with near infrared contrast agents

Siavash Yazdanfar, Chulmin Joo, Chun Zhan, Mikhail Berezin, Walter J. Akers, Samuel Achilefu

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Journal of Biomedical Optics, Vol. 15, Issue 3, 030505 (May 2010). https://doi.org/10.1117/1.3420209

Abstract

While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Siavash Yazdanfar, Chulmin Joo, Chun Zhan, Mikhail Berezin, Walter J. Akers, and Samuel Achilefu "Multiphoton microscopy with near infrared contrast agents," Journal of Biomedical Optics 15(3), 030505 (1 May 2010). https://doi.org/10.1117/1.3420209

Published: 1 May 2010

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