1 May 2010 Virtual four-dimensional imaging of lung parenchyma by optical coherence tomography in mice
Author Affiliations +
J. of Biomedical Optics, 15(3), 036016 (2010). doi:10.1117/1.3425654
In this feasibility study, we present a method for virtual 4-D imaging of healthy and injured subpleural lung tissue in the ventilated mouse. We use triggered swept source optical coherence tomography (OCT) with an A-scan frequency of 20 kHz to image murine subpleural alveoli during the inspiratory phase. The data acquisition is gated to the ventilation pressure to take single B-scans in each respiration cycle for different pressure levels. The acquired B-scans are combined off-line into one volume scan for each pressure level. The air fraction in healthy lungs and injured lungs is measured using 2-D OCT en-face images. Upon lung inspiration from 2 to 12 cmH2O ventilation pressure, the air fraction increases in healthy lungs by up to 11% and in injured lungs by 8%. This expansion correlates well with results of previous studies, reporting increased alveolar area with increased ventilation pressures. We demonstrate that OCT is a useful tool to investigate alveolar dynamics in spatial dimensions.
Sven Meissner, Arata Tabuchi, Michael Mertens, Wolfgang Michael Kuebler, Edmund Koch, "Virtual four-dimensional imaging of lung parenchyma by optical coherence tomography in mice," Journal of Biomedical Optics 15(3), 036016 (1 May 2010). https://doi.org/10.1117/1.3425654


Optical coherence tomography

3D acquisition

In vivo imaging

3D image processing


Data acquisition

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