1 May 2010 Assessing delivery and quantifying efficacy of small interfering ribonucleic acid therapeutics in the skin using a dual-axis confocal microscope
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Abstract
Transgenic reporter mice and advances in imaging instrumentation are enabling real-time visualization of cellular mechanisms in living subjects and accelerating the development of novel therapies. Innovative confocal microscope designs are improving their utility for microscopic imaging of fluorescent reporters in living animals. We develop dual-axis confocal DAC microscopes for such in vivo studies and create mouse models where fluorescent proteins are expressed in the skin for the purpose of advancing skin therapeutics and transdermal delivery tools. Three-dimensional image volumes, through the different skin compartments of the epidermis and dermis, can be acquired in several seconds with the DAC microscope in living mice, and are comparable to histologic analyses of reporter protein expression patterns in skin sections. Intravital imaging with the DAC microscope further enables visualization of green fluorescent protein GFP reporter gene expression in the skin over time, and quantification of transdermal delivery of small interfering RNA siRNA and therapeutic efficacy. Visualization of transdermal de-livery of nucleic acids will play an important role in the development of innovative strategies for treating skin pathologies.
© (2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
Hyejun Ra, Emilio Gonzalez-Gonzalez, Bryan R. Smith, Sanjiv Sam Gambhir, Gordon S. Kino, Olav Solgaard, Roger L. Kaspar, Christopher H. Contag, "Assessing delivery and quantifying efficacy of small interfering ribonucleic acid therapeutics in the skin using a dual-axis confocal microscope," Journal of Biomedical Optics 15(3), 036027 (1 May 2010). https://doi.org/10.1117/1.3432627 . Submission:
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