1 May 2010 Quantitative Raman spectroscopy in turbid media
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Abstract
Intrinsic Raman spectra of biological tissue are distorted by the influences of tissue absorption and scattering, which significantly challenge signal quantification. A combined Raman and spatially resolved reflectance setup is introduced to measure the absorption coefficient µa and the reduced scattering coefficient µ of the tissue, together with the Raman signals. The influence of µa and µ on the resonance Raman signal of -carotene is measured at 1524 cm−1 by tissue phantom measurements and Monte Carlo simulations for µa=0.01 to 10 mm−1 and µ=0.1 to 10 mm−1. Both methods show that the Raman signal drops roughly proportional to 1/µa for µa>0.2 mm−1 in the measurement geometry and that the influence of µ is weaker, but not negligible. Possible correction functions dependent on the elastic diffuse reflectance are investigated to correct the Raman signal for the influence of µa and µ, provided that µa and µ are measured as well. A correction function based on the Monte Carlo simulation of Raman signals is suggested as an alternative. Both approaches strongly reduce the turbidity-induced variation of the Raman signals and allow absolute Raman scattering coefficients to be determined.
© (2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
Carina Reble, Ingo H. Gersonde, Stefan Andree, Hans-Joachim Eichler, Jürgen Helfmann, "Quantitative Raman spectroscopy in turbid media," Journal of Biomedical Optics 15(3), 037016 (1 May 2010). https://doi.org/10.1117/1.3456370 . Submission:
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