1 July 2010 Transient transfection of mammalian cells using a violet diode laser
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Abstract
We demonstrate the first use of the violet diode laser for transient mammalian cell transfection. In contrast to previous studies, which showed the generation of stable cell lines over a few weeks, we develop a methodology to transiently transfect cells with an efficiency of up to ~40%. Chinese hamster ovary (CHO-K1) and human embryonic kidney (HEK293) cells are exposed to a tightly focused 405-nm laser in the presence of plasmid DNA encoding for a mitochondrial targeted red fluorescent protein. We report transfection efficiencies as a function of laser power and exposure time for our system. We also show, for the first time, that a continuous wave laser source can be successfully applied to selective gene silencing experiments using small interfering RNA. This work is a major step towards an inexpensive and portable phototransfection system.
© (2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
Maria Leilani Y. Torre-Mapa, Maria Leilani Y. Torre-Mapa, Liselotte Veldhuis, Liselotte Veldhuis, Martin Ploschner, Martin Ploschner, Kishan Dholakia, Kishan Dholakia, Frank James Gunn-Moore, Frank James Gunn-Moore, } "Transient transfection of mammalian cells using a violet diode laser," Journal of Biomedical Optics 15(4), 041506 (1 July 2010). https://doi.org/10.1117/1.3430730 . Submission:
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