1 July 2010 In vivo carotid artery closure by laser activation of hyaluronan-embedded gold nanorods
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We prove the first application of near-infrared-absorbing gold nanorods (GNRs) for in vivo laser closure of a rabbit carotid artery. GNRs are first functionalized with a biopolymeric shell and then embedded in hyaluronan, which gives a stabilized and handy laser-activable formulation. Four rabbits undergo closure of a 3-mm longitudinal incision performed on the carotid artery by means of a 810-nm diode laser in conjunction with the topical application of the GNRs composite. An effective surgery is obtained by using a 40-W/cm2 laser power density. The histological and electron microscopy evaluation after a 30-day follow-up demonstrates complete healing of the treated arteries with full re-endothelization at the site of GNRs application. The absence of microgranuloma formation and/or dystrophic calcification is evidence that no host reaction to nanoparticles interspersed through the vascular tissue occurred. The observation of a reshaping and associated blue shift of the NIR absorption band of GNRs after laser treatment supports the occurrence of a self-terminating process, and thus of additional safety of the minimally invasive laser procedure. This study underlines the feasibility of using GNRs for in vivo laser soldering applications, which represents a step forward toward the introduction of nanotechnology-based therapies in minimally invasive clinical practices.
© (2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
Paolo Matteini, Paolo Matteini, Fulvio Ratto, Fulvio Ratto, Francesca Rossi, Francesca Rossi, Giacomo Rossi, Giacomo Rossi, Giuseppe Esposito, Giuseppe Esposito, Alfredo Puca, Alfredo Puca, Alessio Albanese, Alessio Albanese, Giulio Maira, Giulio Maira, Roberto Pini, Roberto Pini, } "In vivo carotid artery closure by laser activation of hyaluronan-embedded gold nanorods," Journal of Biomedical Optics 15(4), 041508 (1 July 2010). https://doi.org/10.1117/1.3449574 . Submission:

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