1 July 2010 Silver nanoparticle-induced degranulation observed with quantitative phase microscopy
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Monitoring a degranulation process in a live mast cell is a quite important issue in immunology and pharmacology. Because the size of a granule is normally much smaller than the resolution limit of an optical microscope system, there is no direct real-time live cell imaging technique for observing degranulation processes except for fluorescence imaging techniques. In this research, we propose optical quantitative phase microscopy (QPM) as a new observation tool to study degranulation processes in a live mast cell without any fluorescence labeling. We measure the cell volumes and the cross sectional profiles (x-z plane) of an RBL-2H3 cell and a HeLa cell, before and after they are exposed to calcium ionophore A23187 and silver nanoparticles (AgNPs). We verify that the volume and the cross sectional line profile of the RBL-2H3 cell were changed significantly when it was exposed to A23187. When 50 µg/mL of AgNP is used instead of A23187, the measurements of cell volume and cross sectional profiles indicate that RBL-2H3 cells also follow degranulation processes. Degranulation processes for these cells are verified by monitoring the increase of intracellular calcium ([Ca2+]i) and histamine with fluorescent methods.
© (2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
Wenzhong Yang, Wenzhong Yang, Seungrag Lee, Seungrag Lee, Jiyong Lee, Jiyong Lee, Yoon Sung Bae, Yoon Sung Bae, Dug-Young Kim, Dug-Young Kim, "Silver nanoparticle-induced degranulation observed with quantitative phase microscopy," Journal of Biomedical Optics 15(4), 045005 (1 July 2010). https://doi.org/10.1117/1.3470104 . Submission:

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