1 July 2010 Superresolved digital in-line holographic microscopy for high-resolution lensless biological imaging
Author Affiliations +
Abstract
Digital in-line holographic microscopy (DIHM) is a modern approach capable of achieving micron-range lateral and depth resolutions in three-dimensional imaging. DIHM in combination with numerical imaging reconstruction uses an extremely simplified setup while retaining the advantages provided by holography with enhanced capabilities derived from algorithmic digital processing. We introduce superresolved DIHM incoming from time and angular multiplexing of the sample spatial frequency information and yielding in the generation of a synthetic aperture (SA). The SA expands the cutoff frequency of the imaging system, allowing submicron resolutions in both transversal and axial directions. The proposed approach can be applied when imaging essentially transparent (low-concentration dilutions) and static (slow dynamics) samples. Validation of the method for both a synthetic object (U.S. Air Force resolution test) to quantify the resolution improvement and a biological specimen (sperm cells biosample) are reported showing the generation of high synthetic numerical aperture values working without lenses.
© (2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
Vicente Micó, Vicente Micó, Zeev Zalevsky, Zeev Zalevsky, "Superresolved digital in-line holographic microscopy for high-resolution lensless biological imaging," Journal of Biomedical Optics 15(4), 046027 (1 July 2010). https://doi.org/10.1117/1.3481142 . Submission:
JOURNAL ARTICLE
5 PAGES


SHARE
Back to Top