1 September 2010 Toward surface quantification of liver fibrosis progression
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Monitoring liver fibrosis progression by liver biopsy is important for certain treatment decisions, but repeated biopsy is invasive. We envision redefinition or elimination of liver biopsy with surface scanning of the liver with minimally invasive optical methods. This would be possible only if the information contained on or near liver surfaces accurately reflects the liver fibrosis progression in the liver interior. In our study, we acquired the second-harmonic generation and two-photon excitation fluorescence microscopy images of liver tissues from bile duct-ligated rat model of liver fibrosis. We extracted morphology-based features, such as total collagen, collagen in bile duct areas, bile duct proliferation, and areas occupied by remnant hepatocytes, and defined the capsule and subcapsular regions on the liver surface based on image analysis of features. We discovered a strong correlation between the liver fibrosis progression on the anterior surface and interior in both liver lobes, where biopsy is typically obtained. The posterior surface exhibits less correlation with the rest of the liver. Therefore, scanning the anterior liver surface would obtain similar information to that obtained from biopsy for monitoring liver fibrosis progression.
© (2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
Yuting He, Yuting He, Chiang Huen Kang, Chiang Huen Kang, Shuoyu Xu, Shuoyu Xu, Xiaoye Tuo, Xiaoye Tuo, Scott Trasti, Scott Trasti, Dean C. S. Tai, Dean C. S. Tai, Anju Mythereyi Raja, Anju Mythereyi Raja, Qiwen Peng, Qiwen Peng, Peter T. C. So, Peter T. C. So, Jagath C. Rajapakse, Jagath C. Rajapakse, Roy Welsch, Roy Welsch, Hanry Yu, Hanry Yu, } "Toward surface quantification of liver fibrosis progression," Journal of Biomedical Optics 15(5), 056007 (1 September 2010). https://doi.org/10.1117/1.3490414 . Submission:

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