1 September 2010 Fluorescence lifetime imaging microscopy for brain tumor image-guided surgery
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Abstract
We demonstrate for the first time the application of an endoscopic fluorescence lifetime imaging microscopy (FLIM) system to the intraoperative diagnosis of glioblastoma multiforme (GBM). The clinically compatible FLIM prototype integrates a gated (down to 0.2 ns) intensifier imaging system with a fiber-bundle (fiber image guide of 0.5 mm diameter, 10,000 fibers with a gradient index lens objective 0.5 NA, and 4 mm field of view) to provide intraoperative access to the surgical field. Experiments conducted in three patients undergoing craniotomy for tumor resection demonstrate that FLIM-derived parameters allow for delineation of tumor from normal cortex. For example, at 460±25-nm wavelength band emission corresponding to NADH/NADPH fluorescence, GBM exhibited a weaker florescence intensity (35% less, p-value <0.05) and a longer lifetime GBM-Amean=1.59±0.24 ns than normal cortex NC-Amean=1.28±0.04 ns (p-value <0.005). Current results demonstrate the potential use of FLIM as a tool for image-guided surgery of brain tumors.
© (2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
Yinghua H. Sun, Yinghua H. Sun, Nisa Hatami, Nisa Hatami, Matthew Yee, Matthew Yee, Jennifer E. Phipps, Jennifer E. Phipps, Daniel S. Elson, Daniel S. Elson, Fredric Gorin, Fredric Gorin, Rudolph J. Schrot, Rudolph J. Schrot, Laura Marcu, Laura Marcu, } "Fluorescence lifetime imaging microscopy for brain tumor image-guided surgery," Journal of Biomedical Optics 15(5), 056022 (1 September 2010). https://doi.org/10.1117/1.3486612 . Submission:
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