Measuring hemodynamics in the developing heart tube with four-dimensional gated Doppler optical coherence tomography

Michael W. Jenkins; Lindsy M. Peterson; Shi Gu; Madhusudhana Gargesha; David L. Wilson; Michiko Watanabe; Andrew M. Rollins

doi:10.1117/1.3509382

1 November 2010 Measuring hemodynamics in the developing heart tube with four-dimensional gated Doppler optical coherence tomography

Michael W. Jenkins, Lindsy M. Peterson, Shi Gu, Madhusudhana Gargesha, David L. Wilson, Michiko Watanabe, Andrew M. Rollins

Author Affiliations +

Journal of Biomedical Optics, Vol. 15, Issue 6, 066022 (November 2010). https://doi.org/10.1117/1.3509382

Abstract

Hemodynamics is thought to play a major role in heart development, yet tools to quantitatively assess hemodynamics in the embryo are sorely lacking. The especially challenging analysis of hemodynamics in the early embryo requires new technology. Small changes in blood flow could indicate when anomalies are initiated even before structural changes can be detected. Furthermore, small changes in the early embryo that affect blood flow could lead to profound abnormalities at later stages. We present a demonstration of 4-D Doppler optical coherence tomography (OCT) imaging of structure and flow, and present several new hemodynamic measurements on embryonic avian hearts at early stages prior to the formation of the four chambers. Using 4-D data, pulsed Doppler measurements could accurately be attained in the inflow and outflow of the heart tube. Also, by employing an en-face slice from the 4-D Doppler image set, measurements of stroke volume and cardiac output are obtained without the need to determine absolute velocity. Finally, an image plane orthogonal to the blood flow is used to determine shear stress by calculating the velocity gradient normal to the endocardium. Hemodynamic measurements will be crucial to identifying genetic and environmental factors that lead to congenital heart defects.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Michael W. Jenkins, Lindsy M. Peterson, Shi Gu, Madhusudhana Gargesha, David L. Wilson, Michiko Watanabe, and Andrew M. Rollins "Measuring hemodynamics in the developing heart tube with four-dimensional gated Doppler optical coherence tomography," Journal of Biomedical Optics 15(6), 066022 (1 November 2010). https://doi.org/10.1117/1.3509382

Published: 1 November 2010

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
4 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY