1 February 2011 Drug-loaded biodegradable microspheres for image-guided combinatory epigenetic therapy in cells
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J. of Biomedical Optics, 16(2), 020507 (2011). doi:10.1117/1.3548878
Abstract
We synthesize drug-loaded poly (lactic-co-glycolic acid) (PLGA) microspheres for image-guided combinatory epigenetic therapy in MCF-10A human mammary epithelial cells. LY294002 and Nile Red are encapsulated in microspheres for sustained drug release and fluorescence microscopic imaging. Drug-loaded microspheres target MCF-10A cells through a three-step binding process involving biotinylated antibody, streptavidin, and biotinylated microspheres. LY294002 loaded microspheres and 5-Aza-2-deoxycytidine are applied to MCF-10A cells for combinatory PI3K/AKT inhibition and deoxyribonucleic acid (DNA) demethylation. Our study implies the technical potential of disease targeting and image-guided combinatory epigenetic therapy using drug-loaded multifunctional biodegradable PLGA microspheres.
Ronald X. Xu, Songbo Xu, Tao Zuo, Tim H. Huang, Rulong Shen, Michael F. Tweedle, "Drug-loaded biodegradable microspheres for image-guided combinatory epigenetic therapy in cells," Journal of Biomedical Optics 16(2), 020507 (1 February 2011). http://dx.doi.org/10.1117/1.3548878
Submission: Received ; Accepted
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KEYWORDS
Cancer

Luminescence

Scanning electron microscopy

Image processing

Magnesium

Radiology

Biomedical engineering

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