1 February 2011 Ultracompact alignment-free single molecule fluorescence device with a foldable light path
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J. of Biomedical Optics, 16(2), 025004 (2011). doi:10.1117/1.3548311
Abstract
Instruments with single-molecule level detection capabilities can potentially benefit a wide variety of fields, including medical diagnostics. However, the size, cost, and complexity of such devices have prevented their widespread use outside sophisticated research laboratories. Fiber-only devices have recently been suggested as smaller and simpler alternatives, but thus far, they have lacked the resolution and sensitivity of a full-fledged system, and accurate alignment remains a critical requirement. Here we show that through-space reciprocal optical coupling between a fiber and a microscope objective, combined with wavelength division multiplexing in optical fibers, allows a drastic reduction of the size and complexity of such an instrument while retaining its resolution. We demonstrate a 4×4×18 cm3 sized fluorescence correlation spectrometer, which requires no alignment, can analyze kinetics at the single-molecule level, and has an optical resolution similar to that of much larger microscope based devices. The sensitivity can also be similar in principle, though in practice it is limited by the large background fluorescence of the commonly available optical fibers. We propose this as a portable and field deployable single molecule device with practical diagnostic applications.
Niraj Kumar Singh, Jenu V. Chacko, Suman Nag, Sudipta Maiti, Varun K. A. Sreenivasan, "Ultracompact alignment-free single molecule fluorescence device with a foldable light path," Journal of Biomedical Optics 16(2), 025004 (1 February 2011). http://dx.doi.org/10.1117/1.3548311
Submission: Received ; Accepted
JOURNAL ARTICLE
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KEYWORDS
Luminescence

Fluorescence correlation spectroscopy

Objectives

Molecules

Fiber lasers

Mirrors

Sensors

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