1 February 2011 High-resolution resonant and nonresonant fiber-scanning confocal microscope
Author Affiliations +
J. of Biomedical Optics, 16(2), 026007 (2011). doi:10.1117/1.3534781
Abstract
We present a novel, hand-held microscope probe for acquiring confocal images of biological tissue. This probe generates images by scanning a fiber-lens combination with a miniature electromagnetic actuator, which allows it to be operated in resonant and nonresonant scanning modes. In the resonant scanning mode, a circular field of view with a diameter of 190 μm and an angular frequency of 127 Hz can be achieved. In the nonresonant scanning mode, a maximum field of view with a width of 69 μm can be achieved. The measured transverse and axial resolutions are 0.60 and 7.4 μm, respectively. Images of biological tissue acquired in the resonant mode are presented, which demonstrate its potential for real-time tissue differentiation. With an outer diameter of 3 mm, the microscope probe could be utilized to visualize cellular microstructures in vivo across a broad range of minimally-invasive procedures.
Benno H. W. Hendriks, Walter C. J. Bierhoff, Jeroen J. L. Horikx, Adrien E. Desjardins, Cees A. Hezemans, Gert W. t'Hooft, Gerald W. Lucassen, Nenad Mihajlovic, "High-resolution resonant and nonresonant fiber-scanning confocal microscope," Journal of Biomedical Optics 16(2), 026007 (1 February 2011). http://dx.doi.org/10.1117/1.3534781
Submission: Received ; Accepted
JOURNAL ARTICLE
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KEYWORDS
Microscopes

Objectives

Scanners

Confocal microscopy

Tissues

Reflectivity

Actuators

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