1 April 2011 Intravital microscopy of subpleural alveoli via transthoracic endoscopy
Author Affiliations +
J. of Biomedical Optics, 16(4), 046002 (2011). doi:10.1117/1.3560297
Abstract
Transfer of too high mechanical energy from the ventilator to the lung's alveolar tissue is the main cause for ventilator-induced lung injury (VILI). To investigate the effects of cyclic energy transfer to the alveoli, we introduce a new method of transthoracic endoscopy that provides morphological as well as functional information about alveolar geometry and mechanics. We evaluate the new endoscopic method to continuously record images of focused subpleural alveoli. The method is evaluated by using finite element modeling techniques and by direct observation of subpleural alveoli both in isolated rat lungs as well as in intact animals (rats). The results confirm the overall low invasiveness of the endoscopic method insofar as the mechanical influences on the recorded alveoli are only marginal. It is, hence, a suited method for intravital microscopy in the rat model as well as in larger animals.
David Schwenninger, Hanna Runck, Stefan Schumann, Josef Guttmann, Jorg Haberstroh, Sven Meissner, Edmund Koch, "Intravital microscopy of subpleural alveoli via transthoracic endoscopy," Journal of Biomedical Optics 16(4), 046002 (1 April 2011). http://dx.doi.org/10.1117/1.3560297
Submission: Received ; Accepted
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KEYWORDS
Endoscopy

Lung

Microscopy

Endoscopes

Tissues

Animal model studies

Mechanics

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