1 May 2011 Automatic cytometric device using multiple wavelength excitations
Author Affiliations +
J. of Biomedical Optics, 16(5), 057006 (2011). doi:10.1117/1.3582162
Abstract
Precise identification of eosinophils, basophils, and specific subpopulations of blood cells (B lymphocytes) in an unconventional automatic hematology analyzer is demonstrated. Our specific apparatus mixes two excitation radiations by means of an acousto-optics tunable filter to properly control fluorescence emission of phycoerythrin cyanin 5 (PC5) conjugated to antibodies (anti-CD20 or anti-CRTH2) and Thiazole Orange. This way our analyzer combining techniques of hematology analysis and flow cytometry based on multiple fluorescence detection, drastically improves the signal to noise ratio and decreases the spectral overlaps impact coming from multiple fluorescence emissions.
Nelly Rongeat, Sylvain Ledroit, Laurence Chauvet, Didier Cremien, Alexandra Urankar, Philippe Nerin, Vincent Couderc, "Automatic cytometric device using multiple wavelength excitations," Journal of Biomedical Optics 16(5), 057006 (1 May 2011). http://dx.doi.org/10.1117/1.3582162
Submission: Received ; Accepted
JOURNAL ARTICLE
8 PAGES


SHARE
KEYWORDS
Luminescence

Blood

Hematology

Statistical analysis

Laser sources

Biological research

Scattering

Back to Top