1 June 2011 Ratiometric spectral imaging for fast tumor detection and chemotherapy monitoring in vivo
Author Affiliations +
J. of Biomedical Optics, 16(6), 066007 (2011). doi:10.1117/1.3589299
Abstract
We report a novel in vivo spectral imaging approach to cancer detection and chemotherapy assessment. We describe and characterize a ratiometric spectral imaging and analysis method and evaluate its performance for tumor detection and delineation by quantitatively monitoring the specific accumulation of targeted gallium corrole (HerGa) into HER2-positive (HER2 +) breast tumors. HerGa temporal accumulation in nude mice bearing HER2 + breast tumors was monitored comparatively by a. this new ratiometric imaging and analysis method; b. established (reflectance and fluorescence) spectral imaging; c. more commonly used fluorescence intensity imaging. We also tested the feasibility of HerGa imaging in vivo using the ratiometric spectral imaging method for tumor detection and delineation. Our results show that the new method not only provides better quantitative information than typical spectral imaging, but also better specificity than standard fluorescence intensity imaging, thus allowing enhanced in vivo outlining of tumors and dynamic, quantitative monitoring of targeted chemotherapy agent accumulation into them.
Jae-Youn Hwang, Daniel L. Farkas, Lali K. Medina-Kauwe, Zeev Gross, Harry B. Gray, "Ratiometric spectral imaging for fast tumor detection and chemotherapy monitoring in vivo," Journal of Biomedical Optics 16(6), 066007 (1 June 2011). http://dx.doi.org/10.1117/1.3589299
Submission: Received ; Accepted
JOURNAL ARTICLE
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KEYWORDS
Tumors

Imaging spectroscopy

Luminescence

In vivo imaging

Image classification

Molecules

Tissues

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