1 July 2011 Label-free optical activation of astrocyte in vivo
Author Affiliations +
J. of Biomedical Optics, 16(7), 075003 (2011). doi:10.1117/1.3600774
Abstract
As the most abundant cell type in the central nervous system, astrocyte has been one of main research topics in neuroscience. Although various tools have been developed, at present, there is no tool that allows noninvasive activation of astrocyte in vivo without genetic or pharmacological perturbation. Here we report a noninvasive label-free optical method for physiological astrocyte activation in vivo using a femtosecond pulsed laser. We showed the laser stimulation robustly induced astrocytic calcium activation in vivo and further verified physiological relevance of the calcium increase by demonstrating astrocyte mediated vasodilation in the brain. This novel optical method will facilitate noninvasive physiological study on astrocyte function.
Myunghwan Choi, Taeyun Ku, Kyungsun Choi, Chulhee Choi, Jonghee Yoon, "Label-free optical activation of astrocyte in vivo," Journal of Biomedical Optics 16(7), 075003 (1 July 2011). http://dx.doi.org/10.1117/1.3600774
Submission: Received ; Accepted
JOURNAL ARTICLE
6 PAGES


SHARE
KEYWORDS
Calcium

In vivo imaging

Femtosecond phenomena

Brain

Laser irradiation

Skull

Pulsed laser operation

Back to Top