1 August 2011 Functional laser speckle imaging of cerebral blood flow under hypothermia
Author Affiliations +
J. of Biomedical Optics, 16(8), 086011 (2011). doi:10.1117/1.3610995
Hypothermia can unintentionally occur in daily life, e.g., in cardiovascular surgery or applied as therapeutics in the neurosciences critical care unit. So far, the temperature-induced spatiotemporal responses of the neural function have not been fully understood. In this study, we investigated the functional change in cerebral blood flow (CBF), accompanied with neuronal activation, by laser speckle imaging (LSI) during hypothermia. Laser speckle images from Sprague-Dawley rats (n = 8, male) were acquired under normothermia (37°C) and moderate hypothermia (32°C). For each animal, 10 trials of electrical hindpaw stimulation were delivered under both temperatures. Using registered laser speckle contrast analysis and temporal clustering analysis (TCA), we found a delayed response peak and a prolonged response window under hypothermia. Hypothermia also decreased the activation area and the amplitude of the peak CBF. The combination of LSI and TCA is a high-resolution functional imaging method to investigate the spatiotemporal neurovascular coupling in both normal and pathological brain functions.
Minheng Li, Peng Miao, Yisheng Zhu, Shanbao Tong, "Functional laser speckle imaging of cerebral blood flow under hypothermia," Journal of Biomedical Optics 16(8), 086011 (1 August 2011). http://dx.doi.org/10.1117/1.3610995
Submission: Received ; Accepted


Cerebral blood flow

Laser speckle imaging



Neurovascular coupling


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