1 November 2011 In vivo spectral imaging of different cell types in the small intestine by two-photon excited autofluorescence
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Abstract
Spectrally resolved two-photon excited autofluorescence imaging is used to distinguish different cell types and functional areas during dynamic processes in the living gut. Excitation and emission spectra of mucosal tissue and tissue components are correlated to spectra of endogenous chromophores. We show that selective excitation with only two different wavelengths within the tuning range of a Ti:sapphire femtosecond laser system yields excellent discrimination between enterocytes, antigen presenting cells and lysosomes based on the excitation and emission properties of their autofluorescence. The method is employed for time-lapse microscopy over up to 8 h. Changes of the spectral signature with the onset of photodamage are demonstrated, and their origin is discussed.
© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)
Regina B. Orzekowsky-Schroeder, Bjoern Martensen, Alfred Vogel, Gereon Hüttmann, Antje Klinger, Maike Blessenohl, Andreas Gebert, "In vivo spectral imaging of different cell types in the small intestine by two-photon excited autofluorescence," Journal of Biomedical Optics 16(11), 116025 (1 November 2011). https://doi.org/10.1117/1.3655587 . Submission:
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