1 November 2011 In vivo photoacoustic imaging of chemotherapy-induced apoptosis in squamous cell carcinoma using a near-infrared caspase-9 probe
Author Affiliations +
J. of Biomedical Optics, 16(11), 116026 (2011). doi:10.1117/1.3650240
Abstract
Anti-cancer drugs typically exert their pharmacological effect on tumors by inducing apoptosis, or programmed cell death, within the cancer cells. However, no tools exist in the clinic for detecting apoptosis in real time. Microscopic examination of surgical biopsies and secondary responses, such as morphological changes, are used to verify efficacy of a treatment. Here, we developed a novel near-infrared dye-based imaging probe to directly detect apoptosis with high specificity in cancer cells by utilizing a noninvasive photoacoustic imaging (PAI) technique. Nude mice bearing head and neck tumors received cisplatin chemotherapy (10 mg/kg) and were imaged by PAI after tail vein injection of the contrast agent. In vivo PAI indicated a strong apoptotic response to chemotherapy on the peripheral margins of tumors, whereas untreated controls showed no contrast enhancement by PAI. The apoptotic status of the mouse tumor tissue was verified by immunohistochemical techniques staining for cleaved caspase-3 p11 subunit. The results demonstrated the potential of this imaging probe to guide the evaluation of chemotherapy treatment.
Qiuhong Yang, Shuang Cai, M. Laird Forrest, Huizhong Cui, Xinmai Yang, "In vivo photoacoustic imaging of chemotherapy-induced apoptosis in squamous cell carcinoma using a near-infrared caspase-9 probe," Journal of Biomedical Optics 16(11), 116026 (1 November 2011). http://dx.doi.org/10.1117/1.3650240
Submission: Received ; Accepted
JOURNAL ARTICLE
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KEYWORDS
Tumors

Cell death

Photoacoustic imaging

In vivo imaging

Tissues

Cancer

Control systems

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