1 December 2011 Multifocus confocal Raman microspectroscopy for rapid single-particle analysis
Author Affiliations +
J. of Biomedical Optics, 16(12), 120503 (2011). doi:10.1117/1.3662456
We have developed a multifocus confocal Raman microspectroscopy system that allows simultaneous analyses of ∼80 individual biological or airborne microparticles based on a precise image-guided technique. Multiple individual particles adhered in random positions on a coverslip were illuminated by a multifocus excitation pattern formed by rapidly steering a single laser beam with a pair of galvo-mirrors, and their Raman scatterings were synchronously projected with another galvo-mirror to different rows of a CCD chip for parallel spectroscopic analyses. We show that this technique can be used to rapidly identify single airborne particles or bacteria collected on a slide and to monitor germination dynamics of multiple bacterial spores in real-time.
Lingbo Kong, Pengfei Zhang, Yong-qing Li, Peter Setlow, "Multifocus confocal Raman microspectroscopy for rapid single-particle analysis," Journal of Biomedical Optics 16(12), 120503 (1 December 2011). http://dx.doi.org/10.1117/1.3662456
Submission: Received ; Accepted

Raman spectroscopy


Imaging spectroscopy

Confocal microscopy

Biological research

Atmospheric particles

Charge-coupled devices

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