1 December 2011 Lensless multispectral digital in-line holographic microscope
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Abstract
An compact multispectral digital in-line holographic microscope (DIHM) is developed that emulates Gabor's original holographic principle. Using sources of varying spatial coherence (laser, LED), holographic images of objects, including optical fiber, latex microspheres, and cancer cells, are successfully captured and numerically processed. Quantitative measurement of cell locations and percentage confluence are estimated, and pseudocolor images are also presented. Phase profiles of weakly scattering cells are obtained from the DIHM and are compared to those produced by a commercially available off-axis digital holographic microscope.
© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)
James P. Ryle, John T. Sheridan, Susan McDonnell, "Lensless multispectral digital in-line holographic microscope," Journal of Biomedical Optics 16(12), 126004 (1 December 2011). https://doi.org/10.1117/1.3659681 . Submission:
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