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1 December 2011 Myofibrillogenesis in live neonatal cardiomyocytes observed with hybrid two-photon excitation fluorescence-second harmonic generation microscopy
Honghai Liu, Wan Qin, Zhen Ma, Bruce Z. Gao, Yonghong Shao, Tong Ye, Thomas K. Borg
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Journal of Biomedical Optics, Vol. 16, Issue 12, 126012 (December 2011). https://doi.org/10.1117/1.3662457
Abstract
We developed a hybrid two-photon excitation fluorescence-second harmonic generation (TPEF-SHG) imaging system with an on-stage incubator for long-term live-cell imaging. Using the imaging system, we observed the addition of new sarcomeres during myofibrillogenesis while a cardiomyocyte was spreading on the substrate. The results suggest that the TPEF-SHG imaging system with an on-stage incubator is an effective tool for investigation of dynamic myofibrillogenesis.
© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2011/16(12)/126012/4/$25.00
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Honghai Liu, Wan Qin, Zhen Ma, Bruce Z. Gao, Yonghong Shao, Tong Ye, and Thomas K. Borg "Myofibrillogenesis in live neonatal cardiomyocytes observed with hybrid two-photon excitation fluorescence-second harmonic generation microscopy," Journal of Biomedical Optics 16(12), 126012 (1 December 2011). https://doi.org/10.1117/1.3662457
Published: 1 December 2011
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Cited by 14 scholarly publications.
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KEYWORDS
Imaging systems
Second-harmonic generation
Harmonic generation
Microscopes
Microscopy
Luminescence
Beam splitters
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