1 February 2011 Forward-collected simultaneous fluorescence lifetime imaging and coherent anti-Stokes Raman scattering microscopy
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Abstract
We demonstrate the simultaneous collection and separation of femtosecond-laser-based forward-collected coherent anti-Stokes Raman scattering (F-CARS) and two-photon-excitation-induced fluorescence lifetime images (FLIM) using time-correlated single photon counting (TCSPC). We achieve this in a nondescanned geometry using a single multimode fiber without significant loss of light, field of view, and most importantly, TCSPC timing fidelity. In addition to showing the ability to separate CARS images from FLIM images using time gating, we also demonstrate composite multimodal epicollected FLIM imaging with fiber-collected F-CARS imaging in live cells.
© (2011) Society of Photo-Optical Instrumentation Engineers (SPIE)
Aaron D. Slepkov, Aaron D. Slepkov, Andrew Ridsdale, Andrew Ridsdale, Adrian F. Pegoraro, Adrian F. Pegoraro, Douglas J. Moffatt, Douglas J. Moffatt, John P. Pezacki, John P. Pezacki, Albert Stolow, Albert Stolow, Huei-Ning Wan, Huei-Ning Wan, Ming-Hao Wang, Ming-Hao Wang, Fu-Jen Kao, Fu-Jen Kao, } "Forward-collected simultaneous fluorescence lifetime imaging and coherent anti-Stokes Raman scattering microscopy," Journal of Biomedical Optics 16(2), 021103 (1 February 2011). https://doi.org/10.1117/1.3490641 . Submission:
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